High throughput bio-imaging and design a cell imaging experiment, M. Tardivel.
During this semester, we will cover the concepts of advanced high throughput analysis bio-imaging in order to image and quantify biological processes in living cells.
Description: This course will provide a large overview of existing technique in the domain of light microscopy: Theory, components, advantages and drawbacks of each modality and their concrete application.
1- Diversity of Modern Microscopy
2- Different Contrast Methods
3- How to Design a Microscopy Experience
4- Understanding a Microscopy Experience.
PRACTICAL WORK
Slide scanning and spinning disk techniques for high throughput 3D bio-imaging, M. Tardivel, A. Bongiovanni (2 days)AIM:
These courses will provide basis in multi-scale optic microscopy. From whole labeled tissue to 3D high-resolution structures, students will acquire the methodology for data acquisition and data analysis with a gold standard 3D software.
Evaluation: Describe parameters to set up a slide scanner and spinning disk acquisition. Describe each technique. Depending of the sample, describe the process for a 3D analysis using Imaris software.
Scientific background :
Beneficial Effect of a Selective Adenosine A2A Receptor Antagonist in the APPswe/PS1dE9 Mouse Model of Alzheimer’s Disease
Front Mol Neurosci. 2018; 11: 235. PMID: 30050407
Emilie Faivre,1 Joana E. Coelho,2 Katja Zornbach,3 Enas Malik,4 Younis Baqi,4,5 Marion Schneider,4 Lucrezia Cellai,1 Kevin Carvalho,1 Shéhérazade Sebda,6 Martin Figeac,6 Sabiha Eddarkaoui,1 Raphaëlle Caillierez,1Yijuang Chern,7 Michael Heneka,8,9 Nicolas Sergeant,1 Christa E. Müller,4 Annett Halle,3,8 Luc Buée,1 Luisa V. Lopes,2 and David Blum1,*
- Enseignant: Meryem Tardivel